Study Shows Taking Sustamine® L-Alanyl-L-Glutamine During Strenuous Exercise May Ward Off Exhaustion Longer

Times to fatigue increased with Sustamine® versus no hydration*

A University of Central Florida (UCF) study showed taking Sustamine® L-Alanyl-L-Glutamine during strenuous exercises may increase endurance and lengthen time to fatigue.1

The study, published June 22, 2015 in the Journal of American College of Nutrition, included 12 male endurance athletes who performed four endurance trials – each consisting of one-hour treadmill runs at 75% VO2 peak (milliliters of oxygen per body weight per minute moved or utilized) followed by a run to exhaustion at 90% VO2 peak. View the study »

One trial allowed no hydration, with another consisted of consumption of a commercial sports drink (1L). The other two trials included low (600mg/L) and high (2g/L) doses of Sustamine® in the sports drink. Participants drank 250ml every 15 minutes. Plasma glutamine, glucose, electrolytes, and osmolality were measured prior to each run, and at 30-, 45-, and 60-minutes.

Sustamine® is a dipeptide of glutamine that provides several substantial benefits such as enhanced recovery, immune system support, and increased metabolic rate. On top of these benefits, research suggests that Sustamine® is absorbed more than 200 percent better than standard L-glutamine.2 This means you get better results while having to use less.

Time to exhaustion was significantly longer during the low- and high-dose Sustamine® trials compared to no hydration. There was no difference in time to exhaustion between sports drink only and no hydration. Plasma glutamine concentrations were significantly elevated at 45-minutes in the Sustamine trials, and remained elevated at 60 minutes during the high-dose test. Sodium concentrations increased from the beginning of exercise and remained stable for the duration of the one-hour run. At 60 minutes, plasma sodium was significantly lower in all trials compared with no hydration.

“Ingestion of Sustamine® [the alanine-glutamine dipeptide] at either the low- or high-dose significantly improved time to exhaustion during high-intensity exercise compared to a no-hydration trial,” said Dr. Jay Hoffman, one of the study scientists and UCF Department of Sport and Exercise Science professor. “For endurance athletes such as long-distance runners, the addition of Sustamine® in hydration protocols may allow for longer times before exhaustion sets in.”


  1. William P. McCormack PhD, Jay R. Hoffman PhD, Gabriel J. Pruna MS, Adam R. Jajtner MS, Jeremy R. Townsend MS, Jeffrey R. Stout PhD, Maren S. Fragala PhD & David H. Fukuda PhD (2015): Effects of l-Alanyl-l-Glutamine Ingestion on One-Hour Run Performance, Journal of the American College of Nutrition, DOI: 10.1080/07315724.2015.1009193
  2. Harris RC, Hoffman JR, Allsopp A, Routledge NB (2012): L-glutamine absorption is enhanced after ingestion of L-alanylglutamine compared with the free amino acid or wheat protein.Nutr Res, 32(4): 272-7. DOI: 10.1016/j.nutres.2012.02.003.

About Sustamine

Sustamine® L-Alanyl-L-Glutamine is a stable dipeptide of L-Alanine and L-Glutamine. Sustamine is more easily absorbed by the body than complex protein molecules. This makes Sustamine a highly effective ingredient for hydration, endurance and recovery.* Vegetarian, allergen-free and the only GRAS L-Alanyl-L-Glutamine, Sustamine is also tasteless, odorless, and stable in liquids.

Sustamine is an ingredient that works on multiple levels to help rehydrate the body and sustain energy levels during exertion. Sustamine combines L-Glutamine (the most important amino acid for stimulating muscle protein synthesis) and L-Alanine (an amino acid needed for rebuilding your body’s glycogen stores). Sustamine enhances performance and recovery in three primary ways. It helps:

  • Replace lost electrolytes and fluids
  • Repair damaged muscle proteins
  • Refill the body’s energy stores

For more information on Sustamine, visit:

About Kyowa Hakko USA

Kyowa Hakko USA is the North American sales office for Kyowa Hakko Bio Co. Ltd., an international health ingredients manufacturer and world leader in the development, manufacturing and marketing of pharmaceuticals, nutraceuticals and food products. Kyowa is the maker of branded ingredients including Cognizin® Citicoline, Pantesin® Pantethine, Setria® Glutathione, as well as Sustamine® L-Alanyl-L-Glutamine.

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